Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific Tris Acetate Ultra Pure, 5 Kilograms
Tris Acetate Ultra Pure is a highly pure buffering agent used for creating stable pH conditions in laboratory applications. This 5-kilogram package of tris(acetate) is particularly useful in electrophoresis applications such as DNA and RNA gel electrophoresis. Its ultra-pure grade ensures that it will not interfere with sensitive experiments, making it ideal for molecular biology procedures requiring precise pH control. The tris acetate buffer is widely used in buffer systems for gel electrophoresis, enzymatic reactions, and other applications where stable buffering is critical for optimal results. Its high purity makes it ideal for research applications that demand reliability and accuracy.
Epigentek Inc TET ASSAY BUFFER (6ML)
The TET Assay Buffer is a component of Epigenase 5mC Hydroxylase TETActivity/Inhibition Assay Kit (Colorimetric)
Teknova DNA Suspension Buffer, pH 8.0. 4L, Sterile, DNase/RNase tested, PCR Grade. 2 pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
eMolecules 850568-79-5 | 5-Boc-amino-2-hydroxymethylphenylboronic acid, dehydrate | Combi-Blocks | MFCD06659853 | 249.070 | C12H16BNO4 | 98.000 | CC(C)(C)OC(=O)Nc1ccc2COB(O)c2c1 | 1g | 117521248
5-Boc-amino-2-hydroxymethylphenylboronic acid, dehydrate | Combi-Blocks | 850568-79-5 | MFCD06659853 | 249.070 | C12H16BNO4 | 98.000 | CC(C)(C)OC(=O)Nc1ccc2COB(O)c2c1 | 1g | 117521248
eMolecules 481681-02-1 | Ambeed | 4-Fluoro-3-(hydroxymethyl)benzeneboronic acid | 250mg | 716529157 | A129856 | MFCD08235075 | 169.95 | C7H8BFO3
Ambeed | NN3-Trimethyl-4-(4455-tetramethyl-132-dioxaborolan-2-yl)benzenesulfonamide | 1g | 719807485 | A1635881 | | MFCD29110016 | 325.230 | C15H24BNO4S
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool<|a>
Boston Bioproducts Inc HBS-N Buffer (10X, pH 7.4) - 4x500ml
HBS-N Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-N Buffer (1X, pH 7.4) - 4x500ml
HBS-N Buffer (1X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Apexbio Technology LLC SalI 4000U
SalI is a recombinant restriction endonuclease targeting the specific double-stranded DNA sequence 5 -GTCGAC-3 It is designed to recognize and cleave this DNA motif enabling DNA fragment digestion and facilitating downstream ligation reactions SalI exerts its biological activity primarily through sequence-specific endonucleolytic cleavage generating cohesive DNA ends In in vitro studies SalI demonstrates effective and rapid digestion of DNA substrates including plasmids PCR amplicons and genomic DNA Based on these properties SalI holds research potential in molecular cloning mapping of genomic regions and preparation of DNA inserts or vectors
Alkali Scientific SSC Buffer 20X Powder, 2635.6 Grams of Powder, Makes 10 Liters of 20X Solution
SSC Buffer 20X Powder (2635.6 Grams) is an ultra-concentrated solution designed for DNA hybridization applications in molecular biology. This large volume of powder allows labs to prepare up to 10 liters of 20X SSC solution, ideal for high-throughput labs or large-scale experiments. SSC Buffer is critical in hybridization protocols, where high ionic strength is needed to support stable binding of nucleic acid probes to their target sequences. It is commonly used in Southern and Northern blotting, in situ hybridization, and other DNA or RNA detection techniques. The 10-liter yield ensures that large-scale or long-term projects are well-supported with this essential reagent.
Boston Bioproducts Inc M9 Minimal Salts Solution - 2x1L
M9 Minimal Salts Solution - 2x1L (For Research Use and Further Manufacturing Only)
Alkali Scientific Saline 0.85% 20mL, 10/Pk
Saline 0.85% is a sterile isotonic sodium chloride solution widely used in microbiology and laboratory testing. The concentration closely matches the osmotic pressure of bacterial and mammalian cells, helping maintain cellular integrity during handling and dilution. Unlike distilled water, which can cause cells to burst or shrink, this balanced saline preserves cell viability and structure. Because the solution contains no nutrients or buffering components, it provides an inert environment that allows microorganisms to remain stable without stimulating growth. The 20mL tube format offers a convenient volume for washing microbial cells, preparing suspensions, or diluting cultures prior to analysis in microbiological testing and quality control procedures.
Alkali Scientific Saline 0.45% 2mL, 10/Pk
Saline 0.45% is a sterile sodium chloride solution commonly referred to as half normal saline. With a salt concentration lower than physiological saline, it is considered hypotonic relative to most biological cells. This property allows water to move into cells through osmosis, causing them to swell under controlled laboratory conditions. Such behavior is useful in certain cytological and cell preparation procedures where swelling assists with cell lysis or chromosome spreading for microscopic examination. The solution is also used for rehydrating freeze dried reagents, enzymes, or antibiotics when lower ionic strength is required. In laboratory workflows it may also serve as a gentle washing or dilution solution in molecular biology and biochemical procedures.
Boston Bioproducts Inc Tris-Base (2 M) - 2x1L
Bioprocess Grade - Tris-Base (2 M) - 2x1L (For Research Use and Further Manufacturing Only)