Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Kd Medical Inc TBS, 20X, PH7.4, 2000ML
20X Tris Buffer Saline is ready for dilution to a 1X working solution. Used in various research applications. One 2 Liter cubitainer.
Kd Medical Inc TRIS-HCL, 1M, PH 7.4, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 7.4. One 500ml bottle.
Growcells PBS 20X 10L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 9.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 9.5 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.5 (0.2 micron filtered), 500 ml.
Seracare Life Sciences Inc 10X TRIS-GLYCINE TRANS BUFFER
10X Tris-glycine Transfer Buffer is intended for Western blotting and gel electrophoresis. It is ideal for preparing a standard Western blot transfer buffer (Towbin) and for use as a gel electrophoresis buffer for native Tris-glycine gel without SDS. pH=8.3. Conductivity controlled.
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer | MFCD00236358 | 4 L
Tris-Borate-EDTA buffer | MFCD00236358 | 4 L
Kd Medical Inc TRIS HCL 1M PH 8.O 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 8.0. One 500ml bottle.
Kd Medical Inc TBE 10X 1 LITER BOTTLE
Tris Borate EDTA is used for polyacrylamide and agarose gel electrophoresis. 10X TBE is concentrated and ready to be diluted to a 1X working solution. One 1 liter bottle.
Kd Medical Inc TRIS BUFFER, 2M, PH 7.5, 500ML
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. Tris Solution, 2M, provides a convenient stock solution for preparing Tris buffers> One 500 ml bottle.
Kd Medical Inc PBS 1X, PH 7.4 W/SOD AZIDE,20L
Phosphate Buffered Saline is used as a wash buffer in several lab applications. Contains sodium azide FACS washing grade, pH 7.4. One 20 liter cubitainer.
Kd Medical Inc MOPS BUFFER 10X, 1L
10 x MOPS Buffer is intended for use at 1x as the electrophoresis and gel running buffer during the separation of RNA on denaturing formaldehyde/agarose gels. One 1 liter bottle.